In Vitro Evaluation of the Nutritional Formula with Local Ingredients in Prevention of Stunting

Document Type : Original Article

Authors

1 Department of Nutrition, Polytechnic Health Ministry of Health, Bengkulu City, Indonesia

2 Department of Nutrition, Polytechnic Health Ministry of Health, Bandung City, Indonesia

3 Department of Chemistry. Faculty of Sciences and mathematics, Dipnegoro University, Semarang, Indonesia

10.30476/ijns.2025.105334.1392

Abstract

Background: Stunting is a global public health issue that negatively impacts children’s physical and cognitive development, particularly during the critical 1,000-day window from conception to two years of age. This study aimed to develop and evaluate the potential of a Formula Diet to Prevent Stunting (FDCS), which was consisted of local ingredients such as kefir spirulina, moringa leaves, turmeric, fish meal, and low-fat high-calcium milk.
Methods: The methodology was an in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) approach to determine the proximate composition analysis, antioxidant activity and phytochemical screening of FDCS 1-3 containing kefir, moringa (Moringa oleifera) leaf, spirulina, Curcuma longa. fishmeal, and low fat high calcium milk.
Results: The inhibition value of samples with a concentration of 100 ppm in formula 1 was 11.27828%; in formula 2 was 8.418694% and in formula 3 was 1.589842%. The inhibition value of samples with a concentration of 1000 ppm in formula 1 was 30.70849%; in formula 2 was 16.53863% and in formula 3 was 8.898848%. The linear equation was y=0.0067x-0.0027, y=0.0067x-0.0027 and y=0.0067x-0.0027, respectively with the coefficient of determination of R2=0.9978R^2=0.9978R2=0.9978, which showed a very strong linear relationship between concentration and absorbance. Important minerals were phosphorus (807.82 mg/kg), zinc (0.77 mg/100 g),
iron (0.50 mg/100 g), and calcium (117.11 mg/100 g).
Conclusion: These findings have important implications for nutritional intervention strategies using locally sourced ingredients to address stunting in Indonesia.

Highlights

Tonny Cortis Maigoda (Google Scholar)

Keywords

References

  1. Unicef. Improving child nutrition, the achievable imperative for global progress. Unicef.; 2013.
  2. Kemenkes ri. Hasil survei status gizi indonesia (ssgi) 2022. Badan kebijakanpembangunan kesehatan kementerian kesehatan republik indonesia jakarta. Published online 2023.
  3. Kamsiah, Yuliantini E, Maigoda TC, et al. The Efficacy of Fishbean Complementary Feeding and Community-Based Total Sanitation on Toddler Feeding Practices in Stunting Prevention. Int J Nutr Sci. 2025;10:1-8. DOI: 10.30476/ijns.2025.105817.1406.
  1. Guerrant RL, Leite AM, Pinkerton R, et al. Biomarkers of environmental enteropathy, inflammation, stunting, and impaired growth in children in northeast brazil. PLoS One. 2016;11:e0158772. DOI:10.1371/journal.pone.0158772. PMID: 27690129.
  2. Masoumi SJ, Mehrabani D, Saberifiroozi M, et al. The effect of yogurt fortified with Lactobacillus acidophilus and Bifidobacterium sp. probiotic in patients with lactose intolerance. Food Sci Nutr. 2021;9:1704-1711. DOI: 10.1002/fsn3.2145. PMID: 33747481.
  3. Kent M, Welladsen HM, Mangott A, et al. Nutritional evaluation of australian microalgae as potential human health supplements. PLoS One. 2015;10:e0118985. DOI:10.1371/journal.pone.0118985. PMID: 25723496.
  4. Mutrikah M, Santoso H, Syauqi A, et al. ’Profil bioaktif pada tanaman temulawak (curcuma xanthorizza roxb) dan beluntas’’ (pluchea indica less) plants. J Ilmiah Biosaintropis (Bioscience-Tropic). 2018;4:15-21. DOI 10.33474/e-jbst.v4i1.143.
  5. Mehrabani D, Farjam M, Geramizadeh B, et al. The healing effect of curcumin on burn wounds in rat. World J Plast Surg. 2015;4:29-35. PMID: 25606474.
  6. Rezaei M, Hassanzadeh Nemati N, et al. Characterization of sodium carboxymethyl cellulose/calcium alginate scaffold loaded with curcumin in skin tissue engineering. J Appl Polymer Sci. 2022;139:52271. DOI: 10.1002/app.52271.
  7. Fitriani F, Roziana R. Analysis Preference between Dried Catfish Noodles and Yellow Pumkin Flour Substitution. Int J Nutr Sci. 2023;8:57-62. DOI: 10.30476/IJNS.2023.96987.1204.
  8. International food policy research institute. From promise to impact ending malnutrition by 2030. Ifpri; 2016.
  9. Nurcholis W, Sya’bani Putri DN, Husnawati H, et al. Total flavonoid content and antioxidant activity of ethanol and ethyl acetate extracts from accessions of amomum compactum fruits. Ann Agric Sci. 2021;66:58-62. DOI:10.1016/j.aoas.2021.04.001.
  10. Erkekoglou I, Nenadis N, Samara E, et al. Functional teas from the leaves of arbutus unedo: phenolic content, antioxidant activity, and detection of efficient radical scavengers. Plant Foods Hum Nutr. 2017;72:176-183. DOI:10.1007/s11130-017-0607-4. PMID: 28421300.
  11. Hanif F, Berawi KN. Literature review: daun kelor (moringa oleifera) sebagai makanan sehat pelengkap nutrisi 1000 hari pertama kehidupan. J Kesehatan. 2022;13:398-407. DOI:10.26630/jk.v13i2.1415.
  12. Agdeppa IA, Zamora JAT. ’The effects of coconout skim milk and coco-dairy milk blend on the nutritional status of schoolchildren. J Nutr Metab. 2022:2022:6793866. DOI: 10.1155/2022/6793866.
  13. Mehrabani D, Mehrvarz S, Rabiee M. The Effect of Aqueous and Hydroalcoholic Extracts

of the Mango Fruit on Development of Embryonic Tissues during Pregnancy. Int J Nutr Sci. 2019;4:202-205. DOI: 10.30476/IJNS.2019.83956.1040.

  1. Ahmadi S, Firoozi D, Masoumi SJ. The Effect of Micronutrients on COVID-19 Disease: A

Review of Available Evidences. Int J Nutr Sci. 2022;7:10-18. DOI: 10.30476/IJNS.2022.94162.1169.

  1. Davoudzadeh N, Masoumi SJ, Nouri M, et al. Association of Pro-Healthy Diet Index and Non-Healthy Diet Index with Body Composition: Baseline Results from Cohort Study. Int J Nutr Sci. 2025;10:136-145. DOI: 10.30476/ijns.2025.101220.1293.
  1. Farjam M, Mehrabani D, Abbassnia F, et al. The healing effect of Curcuma longa on liver in experimental acute hepatic encephalopathy of rat. Comp Clin Pathol. 2014;23:1669-73. DOI: 10.1007/s00580-014-1883-0.
  2. Panjehshahin MR, Owji AA, Mehrabani D, et al. Effect of curcumin on cholesterol gall-stone induction in rats. J Appl Anim Res. 2003;23:75-80. DOI: 10.1080/09712119.2003.9706771.
  3. Purnomo RB, Tari AIN, Asmoro NW. Variasi penambahan serbuk daun kelor (moringa oleifera) terhadap karakteristik kimiawi fruit leather nanas (ananas comosus (l.)Merr.). Agrisaintifika: J Ilmu-Ilmu Pertanian. 2020;4:60. DOI:10.32585/ags.v4i1.845.
  4. Nugroho MR, Sasongko RN, Kristiawan M. Faktor-faktor yang mempengaruhi kejadian stunting pada anak usia dini di indonesia. J Obsesi: J Pendidikan Anak Usia Dini. 2021;5:2269-2276. DOI:10.31004/obsesi.v5i2.1169.
  5. Trihono T, Atmarita A, Tjandrarini DH, et al. Pendek (stunting) di indonesia, masalah dan solusinya. Lembaga penerbit badan litbangkes; 2015.
  6. Oguzkan SB, Guroy BK, Tonus SS, et al. kilic hi. The bioactive component and DNA protective capability of cultured Spirulina in Turkey (Marmara Region). Genet Aquat Org. 2018;2:7-12. DOI:10.4194/2459-1831-v2_1_02.
  7. Suriawati J, Rachmawati SR. Aktivitas antioksidan ekstrak daun kelor metode dpph dan frap sebagai sediaan obat dan makanan. Medical Sains : J Ilmiah Kefarmasian. 2023;8:253-262. Doi:10.37874/ms.v8i1.512.
  8. Devy kartika sari, anita rahmiwati, rostika flora. Policy brief effectiveness of specific nutrition intervention programs as efforts to prevent stunting in indonesia: literature review. Media publikasi promosi kesehatan indonesia (mppki). 2024;7(6):1446-1450. DOI:10.56338/mppki.v7i6.5247
  9. Hasanuddin I, Purnama ALJ, Sulaeman S, et al. Edukasi tentang pemanfaatan daun kelor (moringa oleifera) guna pencegahan stunting di desa cenrana kec panca lautang. J Kreativitas Pengabdian Kepada Masyarakat (PKM). 2022;5:2458-2466. DOI:10.33024/jkpm.v5i8.6418.
  10. King JC, Brown KH, Gibson RS, et al. Biomarkers of nutrition for development (bond)—zinc review. J Nutr. 2016;146:858s-885s. DOI:10.3945/jn.115.220079. PMID: 26962190.
  11. Rabbani Haghighi N, Naghsh N, et al. The comparison of pretreatment effects of boiled coffee and curcuruma longa on serum albumin as a liver indicator in male rats injected with tioacetamide. J Adv Biomed Sci. 2014;4:58-66.
  12. Malde MK, Graff IE, Siljander-rasi H, et al. Original article: fish bones - a highly available calcium source for growing pigs. J Anim Physiol Anim Nutr (Berl). 2010;94:e66-e76. DOI:10.1111/j.1439-0396.2009.00979.x.
  13. Kusumawardani H, Juwantoro D, Ayuni P, Samsudin M. Vegetable substituted tuna nuggets and changes in nutrient content during frozen storage. IOP Conference Series: Earth Environ Sci. 2023;1200:012004. DOI:10.1088/1755-1315/1200/1/012004.
  14. Fikawati S, Syafiq A, Ririyanti RK, et al. Energy and protein intakes are associated with stunting among preschool children in central jakarta, indonesia: a case-control study. Malays J Nutr. 2021;27:081-091. DOI:10.31246/mjn-2020-0074.
  15. Pramono MA, Ningtyias FW, Rohmawati N, et al. Pengaruh penambahan tepung daun kelor [moringa oleifera] terhadap kadar protein, kalsium, dan daya terima nugget ikan lemuru [sardinella lemuru]. Penelitian gizi dan makanan :J Nutr Food Res. 2021;44:1-10. DOI:10.22435/pgm.v44i1.2639.
  16. Ardian IL, Puspareni ID, Fauziyah A, et al. Analisis kandungan gizi dan daya terima cookies berbahan dasar tepung bekatul dan tepung ikan tuna untuk balita gizi kurang. J Nutr College. 2022;11:42-50. DOI:10.14710/jnc.v11i1.31177.
  17. Syandri h. The nutritional content of bilih fish (mystacoleucus padangensis blkr) eggs waste as source of fish feed material. J Akuakultur Indonesia. 2011;10:74. DOI:10.19027/jai.10.74-80
  18. Adawyah R, khotiffah SK, Wahyudinur, et al. Pengaruh lama pemasakan terhadap kadar protein, lemak, profil asam amino, dan asam lemak tepung ikan sepat rawa (trichogaster trichopterus). J Pengolahan Hasil Perikanan Indonesia. 2020;23:286-294. DOI:10.17844/jphpi.v23i2.32339
  19. Gunawan G, Hutapea JH, Setiadi A, et al. Perkembangan saluran dan sistem pencernaan pada larva ikan tuna sirip kuning, thunnus albacares (bonnaterre, 1788). J Riset Akuakultur. 2019;13:309. DOI:10.15578/jra.13.4.2018.309-316
  20. Garwan R, Kusumaningrum H, Nurhayati T, et al. Karakterisasi jeroan ikan cakalang sebagai skrining awal bahan baku perangkap lalat rumah musca domestica dan antibakteri. J Pengolahan Hasil Perikanan Indonesia. 2022;25:34-51. DOI:10.17844/jphpi.v25i1.38555
  21. Utomo NBP, Susan , Setiawati M. Role of various fishmeal ingredients on sangkuriang catfish clarias sp. Growth. J Akuakultur Indonesia. 2015;12:158. DOI:10.19027/jai.12.158-168.
  22. Matondo FK, Takaisi K, Nkuadiolandu AB, et al. Spirulina supplements improved the nutritional status of undernourished children quickly and significantly: experience from kisantu, the democratic republic of the congo. Int J Pediatr. 2016;2016:1-5. DOI:10.1155/2016/1296414.
International Journal of Nutrition Sciences
Volume 10, Issue 4 - Serial Number 4
December 2025
Pages 601-610

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