Document Type : Original Article
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demonstrated that they have benefcial effects in the treatment of many diseases. Herein, we developed a simpler and effective separation method of ﬂavones and isoﬂavones from S. japonica fruit by one-step HSCCC based on the COSMO-RS model.
Methods: HSCCC was applied for the preparative separation and purifcation of fve active compounds from S. japonica L. fruit (three ﬂavone glycosides and two isoﬂavone glycosides). Under the assistance of a COSMO-RS, one-step separation with the two-elution solvent system that was designed.
Results: The frst elution was conducted with the lower phase of ethyl acetate-n-butanol-water (5:1:5, v/v), and the second elution was performed with the same one containing 4.0 mmol L-1 of NH3·H2O. From 200 mg of crude extract, 11.2 mg of kaempferol-3-O sophoroside (I), 3.2 mg of rutin (II), 3.4 mg of kaempferol-3-O-α-L-ramnopyranosyl-(1–6)-β-Dglucopyranoside (III), 11.5 mg of sophorabioside (IV), and 10.1 mg of sophoricoside (V) were obtained. Their purity values were 98.1%, 97.6%, 99.5%, 99.2%, and 98.3%, respectively.
Conclusion: The fve targets of I-V can be used as reference substances for chromatographic purposes as well as for the further physiological studies. The developed method is instructive for the separation of other ﬂavonoids too.