Comparison of IC50 Antioxidant Analysis of Local Soybean Tempeh and Imported Soybean Tempeh in Indonesia

Widiany, Fery Lusviana; Metty, Metty; Widaryanti, Rahayu; Azizah, Shafira Nur

doi:10.30476/ijns.2022.97526.1213

Comparison of IC50 Antioxidant Analysis of Local Soybean Tempeh and Imported Soybean Tempeh in Indonesia

Document Type : Short Communication

Authors

Fery Lusviana Widiany ¹

Metty Metty ²

Rahayu Widaryanti ³

Shafira Nur Azizah ²

¹ Department of Nutrition, Faculty of Health Science, Universitas Respati Yogyakarta, Indonesia

² Department of Nutrition, Universitas Respati Yogyakarta, Yogyakarta, Indonesia

³ Department of Midwifery, Faculty of Health Science, Universitas Respati Yogyakarta

10.30476/ijns.2022.97526.1213

Abstract

Background: Antioxidants contain in tempeh are free radical scavengers that can inhibit the process of lipid oxidation and prevent several diseases. Types of tempeh sold in the Indonesian market include local soybean tempeh and imported soybean tempeh. This study aims to compare and analyze the differences in the antioxidant activity of local soybean tempeh
and imported soybean tempeh.
Methods: The research was conducted from June to November 2022 in Yogyakarta, Indonesia. This study used objects in the form of local soybean tempeh and imported soybean tempeh. Tempeh was floured first, then its antioxidant activity was analyzed in the laboratory. Antioxidant activity was defined as the amount of IC50 antioxidant in tempeh flour studied in units of mg/mL, with a ratio scale. IC50 antioxidant analysis used the diphenylpicrylhydrazyl (DPPH) method. The data obtained were compared and analyzed statistically using the Independent t-test.
Results: It was shown that the IC50 antioxidant level in both types of tempeh was significantly different (p=0.0001). The difference in the mean of the two groups showed a higher IC50 antioxidant score in local soybean tempeh of 9.96 mg/mL.
Conclusion: Based on the IC50 antioxidant value, imported soybean tempeh had significantly a higher antioxidant activity than the local soybean tempeh.

Keywords

Antioxidant activity

Antioxidant IC50

Imported soybean tempeh

Local soybean tempeh